Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

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Origins and Dispersal of Cultivated Vanilla (Vanilla planifolia Jacks. [Orchidaceae])

Origins and Dispersal of Cultivated Vanilla (Vanilla planifolia Jacks. [Orchidaceae]). Vanilla is a clonally propagated crop originating from Mesoamerica. Information regarding the circumstances under which vanilla cultivation began is incomplete. Presumably, the Totonac people of Papantla (north-central Veracruz, Mexico) were the earliest to cultivate vanilla; however, the oldest reports of va...

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Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia Andr. : IV. Induction of Vanillic Acid Formation.

Kinetin is used as an elicitor to induce vanillic acid formation in cell suspension cultures of Vanilla planifolia. Maximal induction is observed at a kinetin concentration of 20 micrograms per gram of fresh weight of cells. Vanillic acid synthesis is observed a few hours after elicitation. The effects of kinetin on the activity of some enzymes of the phenylpropanoid pathway, i.e. phenylalanine...

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Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia Andr. : III. Conversion of 4-Methoxycinnamic Acids into 4-Hydroxybenzoic Acids.

Feeding of 4-methoxycinnamic acid, 3,4-dimethoxycinnamic acid and 3,4,5-trimethoxycinnamic acid to cell suspension cultures of Vanilla planifolia resulted in the formation of 4-hydroxybenzoic acid, vanillic acid, and syringic acid, respectively. The homologous 4-methoxybenzoic acids were demethylated to the same products. It is concluded that the side chain degrading enzyme system accepts the 4...

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Fungal pathogens pose a major problem in causing reduction in yield of vanilla crop to considerable level. Fusarium oxysporum, was isolated from naturally infected vanilla plants and an attempt was made to minimize the damage caused by the pathogen using biocontrol agents Trichoderma harzianum, and Pseudomonas fluorescens isolated from soil. The combined inoculation of Trichoderma harzianum wit...

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Metabolic changes in different developmental stages of Vanilla planifolia pods.

The metabolomic analysis of developing Vanilla planifolia green pods (between 3 and 8 months after pollination) was carried out by nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Multivariate data analysis of the (1)H NMR spectra, such as principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA), showed a trend of separation of tho...

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ژورنال

عنوان ژورنال: 3 Biotech

سال: 2017

ISSN: 2190-572X,2190-5738

DOI: 10.1007/s13205-017-0871-x